CYTOCHEMICAL STUDIES

Cytochemical Studies of Mitochondria

1. Mitochondria isolated from rat liver were disrupted with 0.3 per cent deoxycholate and a number of subfractions were isolated from this preparation by differential centrifugation. 2. The protein N, RNA and phospholipide content, as well as the succinoxidase, cytochrome c oxidase, adenylate kinase, and DPNH-cytochrome c reductase of these fractions were determined. 3. Two of these subfraction...

Cytochemical Studies on the Kidney

Determinations of the desoxyribonucleic acid (DNA) content of the kidneys of growing rats were made on the whole tissues and on isolated nuclei. It was found that the DNA content per nucleus is constant in rats of different weights. The DNA content of the kidneys increased proportionately to their mass over the range studied. It is concluded that increase in mass is primarily due to cell multip...

Cytochemical studies with African Horse-Sickness Virus by Fluorescent Microscopy

Cytochemical studies of the vascular endothelium.

Cytochemical methods have been used to examine the vascular endothelium. With hemeproteins and immunocytochemistry, investigators have demonstrated the pathways that blood-borne molecules can take to gain access to the extravascular space (Ghitescu et al. 1986; Milici et al. 1987; Schneeberger and Karnovsky 1971; Simionescu et al. 1975). These same cytochemical methods have also provided eviden...

Cytochemical Studies on the Chicken Erythrocyte

Chicken erythrozytes possess many characteristics which make them a desirable material for experimental studies in cytochemistry, particularly those dealing with the chemical composition of various structural components within the cell. Avian erythrocytes have been used as a convenient source of material for the isolation of nuclei. Investigations along such lines were reported as early as 1869...